T vector for direct selection using green fluorescent protein.

نویسندگان

  • J Kwon
  • K S Park
  • S W Park
  • S Y Choi
چکیده

fied tubulins, p. 478-485. In R.B. Vallee (Ed.), Methods in Enzymology, Vol. 196. Academic Press, New York. 3.Nogales, E., S.G. Wolf and K.H. Downing. 1998. Structure of the αβ tubulin dimer by electron crystallography. Nature 391:199-203. 4.Schiff, P.B., J. Fant and S.B. Horwitz. 1979. Promotion of microtubule assembly in vitro by taxol. Nature 277:665-667. 5.Schiff, P.B. and S.B. Horwitz. 1981. Taxol assembles tubulin in the absence of guanosine 5′-triphosphate or microtubule-associated proteins. Biochemistry 20:3247-3252. 6.Wang, Y.L. 1989. Fluorescent analog cytochemistry: tracing functional protein components in living cells, p. 1-12. In Y.L. Wang and D.L. Taylor (Eds.), Methods in Cell Biology, Vol. 29. Academic Press, New York. 7.Wani, M.C., H.L. Taylor, M.E. Wall, P. Coggon and A.T. McPhail. 1971. Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J. Am. Chem. Soc. 93:2325-2327. This work was supported in part by United States Department of Agriculture Grant No. 94-37304-1227 and National Science Foundation DUE Grant No. 9650222 to M.A.M. Address correspondence to Dr. Melissa A. Melan, Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA. Internet: [email protected]. duq.edu

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عنوان ژورنال:
  • BioTechniques

دوره 25 2  شماره 

صفحات  -

تاریخ انتشار 1998